Affinity purification of synthetic peptides.

Affinity purification of synthetic peptides.

A general strategy and a specific tactic for affinity purification of polypeptides synthesized on solid supports are desbribed and demonstrated. The desired peptide chains were distinguished from terminated peptide chains before removal from the support by attachment of an affinity reagnet (cysteinyl-methionine) bearing an affinity group (thiol) and a binding group (carboxylic acid). After clea...

Synthetic peptides with selective affinity for apoptotic cells.

The appearance of anionic phosphatidylserine (PS) in the outer monolayer of the plasma membrane is a universal indicator of the early/intermediate stages of cell apoptosis. The most common method of detecting PS on a cell surface is to use the protein annexin V; however, in certain applications there is a need for alternative reagents. Recent research indicates that rationally designed zinc 2,2...

Affinity purification

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

Purification of peptides pdf

Purification by Affinity Chromatography

The glycine receptor of rat spinal cord was solubilized with the nonionic detergent Triton X-100 and subsequently purified by affinity chromatography on aminostrychnine-agarose and wheat germ agglutininSepharose. An overall purification of 1950-fold was achieved. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and mercaptoethano1 revealed three glycine receptor-asso...